LOCUS       CHIA-pGEc2              6330 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(5808..5937)
                     /note="3'-ITR"
     misc_feature    complement(5506..5720)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    4958..5499
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4137..4159
                     /note="attB2"
     misc_feature    4937..4942
                     /note="BstB1 site"
     misc_feature    4160..4855
                     /note="Super GFP"
     misc_feature    4856..4906
                     /note="AviTag"
     misc_feature    4907..4930
                     /note="His Tag"
     PCR_frame       3011..4136
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4114
                     /note="OPTIMIZED CHIA coding region"
     misc_feature    4115..4136
                     /note="TEV"
     vector          join(4145..6330,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4144
                     /source="CHIA pDONR"
                     /type="Entry Clone"
     source          1..6330
                     /dnas_title="CHIA-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGTCAGCA CCCCTGAGAA TAGACAGACC TTCATTACCA GCGTGATCAA
     3061 GTTCCTGCGG CAGTACGAGT TCGACGGCCT GGACTTTGAT TGGGAGTACC CTGGCTCTAG
     3121 AGGCAGCCCT CCTCAGGATA AGCACCTGTT TACCGTGCTG GTGCAGGAAA TGAGAGAGGC
     3181 CTTCGAGCAG GAAGCCAAGC AGATCAACAA GCCCAGACTG ATGGTCACAG CTGCCGTGGC
     3241 CGCTGGCATC TCTAATATCC AGAGCGGCTA CGAGATCCCC CAGCTGAGCC AGTACCTGGA
     3301 CTACATCCAC GTGATGACCT ACGACCTGCA CGGCTCTTGG GAGGGCTATA CAGGCGAGAA
     3361 CAGCCCCCTG TACAAGTACC CTACCGATAC CGGCAGCAAC GCCTACCTGA ACGTGGACTA
     3421 TGTGATGAAC TACTGGAAGG ACAATGGCGC CCCTGCCGAG AAGCTGATCG TGGGCTTTCC
     3481 AACCTACGGC CACAACTTCA TCCTGAGCAA CCCCAGCAAT ACCGGAATCG GAGCACCTAC
     3541 ATCTGGCGCT GGACCTGCCG GACCTTATGC CAAAGAAAGC GGCATCTGGG CCTACTATGA
     3601 GATCTGCACC TTCCTGAAGA ACGGCGCCAC ACAGGGATGG GATGCTCCAC AGGAAGTGCC
     3661 TTACGCCTAT CAGGGCAACG TGTGGGTCGG ATACGACAAC ATCAAGAGCT TCGACATCAA
     3721 GGCCCAGTGG CTGAAGCACA ACAAGTTCGG CGGAGCCATG GTCTGGGCCA TCGACCTGGA
     3781 TGATTTCACC GGCACATTCT GTAATCAGGG AAAGTTCCCC CTGATCAGCA CACTGAAGAA
     3841 GGCCCTGGGA CTGCAGAGCG CCTCTTGTAC TGCTCCTGCC CAGCCTATCG AGCCTATTAC
     3901 AGCCGCTCCT TCTGGCAGCG GCAATGGCAG TGGAAGTTCT AGCTCTGGCG GATCTTCTGG
     3961 CGGCTCTGGC TTTTGTGCCG TCAGAGCCAA TGGACTGTAC CCCGTGGCCA ACAACAGAAA
     4021 CGCCTTTTGG CACTGCGTGA ACGGCGTGAC CTACCAGCAG AATTGTCAGG CCGGACTGGT
     4081 GTTCGATACC AGCTGCGATT GCTGCAACTG GGCCgaaaac ttgtactttc aaggctCCAG
     4141 CTTTCTTGTA CAAAGTGGTA TGAGCAAGGG CGAGGAACTG TTCACCGGCG TGGTGCCCAT
     4201 CCTGGTGGAA CTGGATGGCG ACGTGAACGG CCACAAGTTC TCTGTGCGGG GAGAGGGCGA
     4261 AGGCGACGCC ACAAATGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC
     4321 CGTGCCTTGG CCTACCCTCG TGACCACACT GACCTACGGC GTGCAGTGCT TCAGCAGATA
     4381 CCCCGACCAT ATGAAGCGGC ACGACTTCTT CAAGAGCGCC ATGCCCGAGG GCTACGTGCA
     4441 GGAACGGACC ATCAGCTTCA AGGACGACGG CACCTACAAG ACCAGAGCCG AAGTGAAGTT
     4501 CGAGGGCGAC ACCCTCGTGA ACCGGATCGA GCTGAAGGGC ATCGATTTCA AAGAGGACGG
     4561 CAACATCCTG GGCCACAAGC TGGAGTACAA CTTCAACAGC CACAACGTGT ACATCACCGC
     4621 CGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACG TGGAAGATGG
     4681 CAGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGAGATG GCCCCGTGCT
     4741 GCTGCCCGAC AACCACTACC TGAGCACCCA GAGCGTGCTG AGCAAGGACC CCAACGAGAA
     4801 GCGGGACCAC ATGGTGCTGC TGGAATTCGT GACCGCCGCT GGCATCACCC ACGGCATGAG
     4861 CGGCCTGAAC GACATCTTCG AGGCCCAGAA AATCGAGTGG CACGAGCACC ACCATCATCA
     4921 CCATCACCAC TGATGATTCG AAAAATAATA AGGATCCAAT CAACCTCTGG ATTACAAAAT
     4981 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC
     5041 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT
     5101 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG
     5161 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG
     5221 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC
     5281 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT
     5341 GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT
     5401 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG
     5461 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT
     5521 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT
     5581 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG
     5641 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC
     5701 AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA
     5761 GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT
     5821 GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA
     5881 GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT
     5941 TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT
     6001 GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
     6061 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
     6121 CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG
     6181 AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT
     6241 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT
     6301 TTTAACAAAA TATTAACGCT TACAATTTAG
//

